Detection of Yersinia enterocolitica serogroup O:3 by a PCR method
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چکیده
منابع مشابه
A PCR ELISA Method For The Detection Of Yersinia Enterocolitica
and Implications The ELISA assay is a more rapid and sensitive method for PCR product detection than conventional gel electrophoresis. The PCR/ELISA allows for the processing of numerous samples, is relatively inexpensive and eliminates the need for electrophoretic and photographic equipment as well as the use of potential carcinogens such as ethidium bromide which is utilized in the gel electr...
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The ability of Yersinia enterocolitica O3, grown at 25 degrees C, to promote cross-immunity to Y. pestis was lost after repeated subcultures at 37 degrees C, which selected for bacterial populations having lower in vivo survival. Subculturing Y. enterocolitica O3 from 37 to 25 degrees C restored the cross-immunogenicity although the in vivo survival remained low.
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Thisted Lambertz, S. 2005. Development of a PCR-based method for detection of pathogenic Yersinia enterocolitica in pork. Doctor ́s dissertation. ISSN 1652-6880, ISBN 91-576-6922-8 During the last decade, Yersinia enterocolitica has been reported to cause between 500 and 800 cases of human gastroenteritis per year in Sweden. As pigs are the only animals in human food production that regularly ha...
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It is known that Yersinia enterocolitica infection in Japan is caused mainly by serotype O3 biotype 4 strains. Recently, however, a number of serotype O3 strains which were classified biotype 3 and which ferment lactose and xylose, instead of sorbose, and give a negative Voges-Proskauer reaction have been isolated from both humans and animals. In this study, comparisons of four properties were ...
متن کاملDetection of pathogenic Yersinia enterocolitica by a rapid and sensitive duplex PCR assay.
A duplex PCR assay targeting the ail and 16S rRNA genes of Yersinia enterocolitica was developed to specifically identify pathogenic Y. enterocolitica from pure culture. Validation of the assay was performed with 215 clinical Yersinia strains and 40 strains of other bacterial species. Within an assay time of 4 h, this assay offers a very specific, reliable, and inexpensive alternative to the co...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1996
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.34.5.1224-1227.1996